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Then the luciferase assays were carried out using the Dual-Glo Luciferase Assay System (Promega) with a BioTek synergy 4 Multi-Mode Microplate Reader

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Glutamate derived from the glutathione cycle rescues excitatory postsynaptic currents during glutamine limitation We next examined whether glutamate from the glutathione cycle could rescue mEPSC when glutamine supply was restricted (Fig