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2.4 Preparation of paraffin-embedded liver sections Fixed liver tissues (4% PFA, 24 h) were washed with saline and dehydrated using a graded ethanol series (75% → 85% → 90% → 95% → 100% I → 100% II), with each step lasting 0.5–4 h

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A qualitative study of reasons for seeking ceasing opioid substitution treatment in prisons in New South Wales, Australia