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Over 100 years ago, physicians experimented with TPE’s potential to increase lifespan when the first recorded plasma exchange procedure was performed on animals

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In vitro BP incorporation was performed at 37 °C for 1 h, and then, the samples were diluted with sample loading buffer (60 mM Tris-Cl (pH 6.8), 10% glycerol, 2% SDS, 1% β-mercaptoethanol (ME), 0.01% bromophenol blue) and boiled at 100 °C for 10 min